Characterization of Bradyrhizobium spp. Nodulating Lupinus cosentinii and L. luteus Microsymbionts in Morocco

In this work, we analyzed the diversity of nodule-forming bacteria associated with Lupinus luteus and cosentinii grown in Maamora Cork oak forest acidic soils Morocco. The phenotypic analysis showed high strains nodulating two lupine's species. were not tolerant to acidity or alkalinity. They do tolerate salinity temperatures either. isolated from L. more antibiotics than those . plant growth promoting (PGP) activities our are modest, as among 28 tested isolates, only six produced auxins, siderophores, whereas three solubilized phosphates. Only possess activities. rrs gene sequences eight representative selected following ARDRA REP-PCR results revealed that they members genus Bradyrhizobium. Six then retained for further molecular analysis. glnII, recA, gyrB, dnaK , rpoB housekeeping sequence phylogeny some close B. lupini LMG28514 T others may constitute new genospecies Bradyrhizobium unable nodulate Glycine max Phaseolus vulgaris effectively nodulated luteus, cosentinii, angustifolius, Chamaecytisus albidus Retama monosperma nodC nodA symbiotic phylogenies genistearum symbiovar.